Ali, Md. Azahar and Singh, Nawab and Srivastava, Saurabh and Agrawal, Ved V. and John, Renu and Onoda, M. and Malhotra, Bansi D. (2014) Chitosan-Modified Carbon Nanotubes-Based Platform for Low-Density Lipoprotein Detection. Applied Biochemistry and Biotechnology, 174 (3). pp. 926-935. ISSN 0273-2289

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Abstract

We have fabricated an immunosensor based on carbon nanotubes and chitosan (CNT-CH) composite for detection of low density lipoprotein (LDL) molecules via electrochemical impedance technique. The CNT-CH composite deposited on indium tin oxide (ITO)-coated glass electrode has been used to covalently interact with anti-apolipoprotein B (antibody: AAB) via a co-entrapment method. The biofunctionalization of AAB on carboxylated CNT-CH surface has been confirmed by Fourier transform infrared spectroscopic and electron microscopic studies. The covalent functionalization of antibody on transducer surface reveals higher stability and reproducibility of the fabricated immunosensor. Electrochemical properties of the AAB/CNT-CH/ITO electrode have been investigated using cyclic voltammetric and impedimetric techniques. The impedimetric response of the AAB/CNT-CH/ITO immunoelectrode shows a high sensitivity of 0.953 a"broken vertical bar/(mg/dL)/cm(2) in a detection range of 0-120 mg/dL and low detection limit of 12.5 mg/dL with a regression coefficient of 0.996. The observed low value of association constant (0.34 M(-1)s(-1)) indicates high affinity of AAB/CNT-CH/ITO immunoelectrode towards LDL molecules. This fabricated immunosensor allows quantitative estimation of LDL concentration with distinguishable variation in the impedance signal.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Humana Press.
Subjects:	Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Electrochemistry Medical Laboratory Technology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	17 Sep 2015 06:18
Last Modified:	17 Sep 2015 06:18
URI:	http://npl.csircentral.net/id/eprint/1200

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