Puri, Nidhi and Niazib, Asad and Srivastavaa, Avanish Kumar and Rajesh, - (2014) Biointerfacial impedance characterization of reduced graphene oxide supported carboxyl pendant conducting copolymer based electrode. Electrochimica Acta, 123. pp. 211-218. ISSN 0013-4686

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Abstract

We report, a comprehensive physical and biointerfacial electrochemical characteristics of electrodeposited poly(pyrrole-co-pyrrolepropylic acid) (PPy-PPa) copolymer film on the reduced graphene oxide (RGO) sheets attached over a silane modified indium-tin-oxide coated glass, for biosensing applications. The highly specific cardiac myoglobin protein antibody, Ab-cMb, has been covalently immobilized on the copolymer film through its pendent carboxyl group by carbodiimide coupling reaction. The factor 'n' describing divergence of the system from ideal capacitor characteristics exhibits a low value (n = 0.59) in a constant phase element of the impedance. This low value of 'n' showing a porous rough microstructure of PPy-PPa film on RGO exhibiting a diffusive characteristic that has been replaced by dominant charge transfer characteristic (R-et) with n = 0.78 on biomolecular immobilization and subsequent immunoreaction. The bioelectrode exhibits a linear impedance response to human cardiac cMb marker in the range of 10 ng mL(-1) to 1 mu g mL(-1) in phosphate buffer solution (PBS; pH 7.4) at a low frequency region of <10 Hz with a R-et sensitivity of 70.30 Omega cm(2) per decade.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Elsevier.
Uncontrolled Keywords:	Protein antibody Conducting polymer Electron microscopy Graphene oxideAntigena
Subjects:	Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	17 Sep 2015 05:47
Last Modified:	17 Sep 2015 05:47
URI:	http://npl.csircentral.net/id/eprint/1190

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