-, Rajesh and Sharma, Vikash and Tanwar, Vinod Kumar and Biradar, A. M. (2010) Electrochemical Impedance Immunosensor for the Detection of C-Reactive Protein in Aqueous Solution. Sensor Letters, 8 (2). pp. 362-369. ISSN 1546-198X

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Abstract

A label-free electrochemical impedance immunosensor is reported by immobilizing protein antibody, αCRP-Ab, through a self assembled monolayer (SAM) of 3-aminopropyltriethoxysilane (APTES) using a cross linker, Bis[sulfosuccinimidyl] suberate (BS3), on indium tin oxide (ITO) coated glass electrode. The immunosensor (αCRP-Ab/BS3/APTES/ITOglass) was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical techniques. The electrochemical performance of the immunosensor was studied by electrochemical impedance spectroscopy. The results showed an increasing electron-transfer resistance with the immobilization of CRP antibody (αCRP-Ab) on the modified ITO coated glass electrode surface and on their coupling with protein CRP antigen (αCRP-Ag) at the immunosensor surface in the presence of [Fe(CN)6]3−/4− as redox probe. The immunosensor exhibits an electrochemical impedance response to antigen, αCRP-Ab, concentrations in a linear range from 8.5 ng to 9.12 μg mL−1 a with a lowest detection limit of 3.5 ng mL−1 antigen.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s American Scientific Publishers.
Uncontrolled Keywords:	Immunosensor; Cyclic Voltammetry; Electrochemical Impedance Spectroscopy; Covalent Immobilization; Immunoassay
Subjects:	Chemistry Electrochemistry Instruments/ Instrumentation Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	12 Apr 2012 07:01
Last Modified:	12 Apr 2012 07:01
URI:	http://npl.csircentral.net/id/eprint/119

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