Patel, Manoj K. and Solanki, Pratima R. and Kumar, Ashok and Khare, Shashi and Gupta, Sunil and Malhotra, Bansi D. (2010) Electrochemical DNA sensor for Neisseria meningitidis detection. Biosensors and Bioelectronics, 25 (12). pp. 2586-2591. ISSN 0956-5663

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Abstract

Meningitis sensor based on nucleic acid probe of Neisseria meningitidis has been fabricated by immobilization of 5′-thiol end labeled single stranded deoxyribonucleic acid probe (ssDNA-SH) onto gold (Au) coated glass electrode. This ssDNA-SH/Au electrode hybridized with the genomic DNA (G-dsDNA/Au) and amplified DNA (PCR-dsDNA/Au) has been characterized using atomic force microscopy (AFM), Fourier transforms infrared spectroscopy (FT-IR) and electrochemical techniques. The ssDNA-SH/Au electrode can specifically detect upto 10–60 ng/μl of G-dsDNA-SH/Au and PCR-dsDNA-SH/Au of meningitis within 60 s of hybridization time at 25 °C by cyclic voltammetry (CV) using methylene blue (MB) as electro-active DNA hybridization indicator. The values of sensitivities of the G-dsDNA-SH/Au and PCR-dsDNA-SH/Au electrodes have been determined as 0.0115 μA/ng cm−2 and 0.0056 μA/ng cm−2, respectively with regression coefficient (R) as 0.999. This DNA bioelectrode is stable for about 4 months when stored at 4 °C.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	ctrA gene; Electrochemical sensor; Gold electrode; Meningitis; N. meningitidis; DNA biosensor
Subjects:	Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	11 Apr 2012 11:48
Last Modified:	11 Apr 2012 11:48
URI:	http://npl.csircentral.net/id/eprint/118

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