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Abstract

Nucleic acid sensor based on polyaniline (PANI) has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClOS4)-doped PANI film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) chemistry. These dsCT-DNA-PANI-ClO4/ITO and PANI-ClO4/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, scanning electron microscopy (SEM) and Fourier-transform-infrared (FTIR) measurements. This disposable dsCT-DNA-PANI-ClO4/ITO bioelectrode, stable for about 4 months, can be used to detect cypermethrin (0.005ppm) and trichlorfon (0.01ppm) in 30 and 60s, respectively.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s John Wiley and Sons.
Uncontrolled Keywords:	nucleic acid sensor; electrochemical; polyaniline; perchlorate; cypermethrin; trichlorfon
Subjects:	Biochemistry & Molecular Biology Electrochemistry Genetics & Heredity Medical Laboratory Technology
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	18 May 2015 08:36
Last Modified:	18 May 2015 08:36
URI:	http://npl.csircentral.net/id/eprint/1121

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