Prabhakar , Nirmal and Singh , Harpal and Malhotra , Bansi D. (2008) Nucleic acid immobilized polypyrrole–polyvinylsulphonate film for Mycobacterium tuberculosis detection. Electrochemistry Communications , 10 (06). pp. 821-826. ISSN 1388-2481

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Abstract

21-mer peptide acid nucleic acid (PNA) probe specific to 16s-23s rRNA spacer region of Mycobacterium tuberculosis has been covalently immobilized on polypyrrole-polyvinylsulphonate film electro-chemically deposited onto indium-tin-oxide (ITO) glass for detection of complementary target by monitoring guanine oxidation and redox indicators (methylene blue and ruthenium complex) up to 0.1 fmole, 0.1 attomole and 1.0 pmole, respectively within 30 s of hybridization time. The peptide nucleic acid immobilized polypyrrole-polyvinylsulphonate electrode can be used for hybridization detection with complementary sequence in heat-shocked genomic DNA and in serum samples containing genomic M. tuberculosis DNA up to 2.5 pg/mu l within about 60 min at 30 degrees C and can be used 8-9 times.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Subjects:	Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	18 May 2015 08:16
Last Modified:	18 May 2015 08:16
URI:	http://npl.csircentral.net/id/eprint/1119

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