Khan, Raju and Dhayal, Marshal (2008) Nanocrystalline bioactive TiO2-chitosan impedimetric immunosensor for ochratoxin-A. Electrochemistry Communications , 10 (3). pp. 492-495. ISSN 1388-2481

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Abstract

Fourier transform infrared (FT-IR) and UV-visible spectroscopy were used to optimize TiO2 concentration in chitosan (CS) to develop a sensitive CS/TiO2 bioactive electrode. Electrochemical impedance spectroscopy (EIS) used to measure electro-activity of these bioactive electrodes associated with enhance oligosaccharide containing –Cdouble bond; length as m-dashO groups from degradation of CS molecules. This matrix has free –NH2 and –OH functional groups due to higher probability of hydrogen and covalent bonding between –OH group in CS molecules with Ti–O–Ti which supported immobilization of rabbit antibodies (IgGs) and proteins. Ochratoxin A (OTA) was detected and showed a linear response up to 10 ng/mL with CS/TiO2 bio-electrode. The OTA detection sensitivity of 7.5 mM TiO2 added CS bioactive electrode was four times higher than only CS.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Subjects:	Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	20 Apr 2015 08:12
Last Modified:	20 Apr 2015 08:12
URI:	http://npl.csircentral.net/id/eprint/1109

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