Raghav, Ruchika and Yadav, Nisha and Tyagi, Gunjan and Jangir, Deepak K. and Mehrotra, Ranjana and Ganesan, R. and Rajagopal, E. S. (2012) Degradation studies of organic acids in commercially packed fruit juices: A reverse phase high performance liquid chromatographic approach. International Journal of Food Engineering, 8 (4). pp. 16-33. ISSN 1556-3758

[img] PDF - Published Version
Restricted to Registered users only

Download (824Kb) | Request a copy


Organic acids are important constituents of fruit juices. They render tartness, flavour and specific taste to fruit juices. Shelf life and stability of fruit juices are important factors, which determine their nutritional quality and freshness. In this view, the effect of storage on the concentration of organic acids in commercially packed fruit juices is studied by reverse phase high performance liquid chromatography (RP-HPLC). Ten packed fruit juices from two different brands are stored at 30 C for 24, 48 and 72 hours. A reverse phase high performance liquid chromatographic method is used to determine the concentration of oxalic, tartaric, malic, ascorbic and citric acid in the fruit juices during storage. The chromatographic analysis of organic acids is carried out using mobile phase 0.5% (w/v) ammonium dihydrogen orthophosphate buffer (pH 2.8) on C18 column with UV-Vis detector. The results show that the concentration of organic acids generally decreases in juices under study with the increase in storage time. All the fruit juices belonging to tropicana brand underwent less organic acid degradation in comparison to juices of real brand. Orange fruit juice is found to be least stable among the juices under study, after the span of 72 hours. Amongst all the organic acids under investigation minimum stability is shown by ascorbic acid followed by malic and citric acid.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s De Gruyter.
Subjects: Food Science & Technology
Depositing User: Users 27 not found.
Date Deposited: 07 Feb 2020 10:25
Last Modified: 07 Feb 2020 10:25
URI: http://npl.csircentral.net/id/eprint/3653

Actions (login required)

View Item View Item