Srivastava, Saurabh and Ali, Md Azahar and Srivastava, Anchal and Umrao, Sima and Parashar, Anchal and Sumana, Gajjala and Malhotra, B. D. and Pandey, Shyam Sudhir and Hayase, Shuji (2014) Graphene Oxide-Based Biosensor for Food Toxin Detection. Applied Biochemistry and Biotechnology, 174 (3). 960-970 . ISSN 0273-2289

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Abstract

We report results of the studies relating to the fabrication of a highly sensitive label free biosensor based on graphene oxide (GO) platform for the detection of aflatoxin B1 (AFB(1)) which is most toxic and predominant food toxin, using electrochemical impedance spectroscopy. The structural and optical characterization of GO/Au and anti-AFB(1)/GO/Au has been done by electron microscopy, Raman, X-ray diffraction (XRD), UV-vis and electrochemical impedance spectroscopy (EIS). The impedimetric sensing response of immunoelectrode as a function of AFB(1) concentration reveals wider linear detection range (0.5-5 ng/ml), high sensitivity (639 a"broken vertical bar ng(-1) ml), improved detection limit (0.23 ng ml(-1)) and good stability (5 weeks) for the label-free detection. Association constant (k (a)) for antigen-antibody interaction obtained as 0.46 ng ml(-1) indicates high affinity.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Humana Press.
Uncontrolled Keywords:	Graphene oxide . Aflatoxin B1 . Immunosensor . Electrochemical impedance spectroscopy
Subjects:	Biochemistry & Molecular Biology Food Science & Technology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	05 Oct 2015 11:17
Last Modified:	05 Oct 2015 11:17
URI:	http://npl.csircentral.net/id/eprint/1311

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