Solanki, Pratima R. and Prabhakar, Nirmal and Pandey, M. K. and Malhotra, B. D. (2008) Nucleic acid sensor for insecticide detection. Journal of Molecular Recognition , 21 (4). pp. 217-223. ISSN 1099-1352

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Nucleic acid sensor based on polyaniline (PANI) has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClOS4)-doped PANI film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) chemistry. These dsCT-DNA-PANI-ClO4/ITO and PANI-ClO4/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, scanning electron microscopy (SEM) and Fourier-transform-infrared (FTIR) measurements. This disposable dsCT-DNA-PANI-ClO4/ITO bioelectrode, stable for about 4 months, can be used to detect cypermethrin (0.005ppm) and trichlorfon (0.01ppm) in 30 and 60s, respectively.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s John Wiley and Sons.
Uncontrolled Keywords: nucleic acid sensor; electrochemical; polyaniline; perchlorate; cypermethrin; trichlorfon
Subjects: Biochemistry & Molecular Biology
Genetics & Heredity
Medical Laboratory Technology
Depositing User: Ms Neetu Chandra
Date Deposited: 18 May 2015 08:36
Last Modified: 18 May 2015 08:36

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