Yadav, Surendra K. and Singh, Jay and Agrawal, Ved Varun and Malhotra, B. D. (2012) Nanostructured nickel oxide film for application to fish freshness biosensor. Applied Physics Letters, 101 (2). 023703-023707. ISSN 0003-6951

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Abstract

Xanthine oxidase (XOx) has been physisorbed onto nanostructured nickel oxide (n-NiO, similar to 41nm), electrophoretically deposited onto indium-tin-oxide (ITO) glass substrate using poly-ethylene glycol via co-precipitation. The electrochemical response studies of the XOx/n-NiO/ITO bioelectrode show linear response in the xanthine concentration range as 10-200 mu M (R-2 = 0.9989), sensitivity as 0.6214 mu A/mu M cm(2), detection limit as 2.989 mu M, standard deviation as 0.6192 mu A, response time of 15 s with the stability of 100 days. The observed low value (0.0306 mM) Michaelis-Menten constant (K-m) indicates high affinity of XOx towards xanthine.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s American Institute of Physics
Subjects:	Applied Physics/Condensed Matter
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 11:06
Last Modified:	19 Aug 2026 11:06
URI:	https://npl.csircentral.net/id/eprint/3475

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