Singh, K. and Solanki, Pratima R. and Basu, Tinku and Basu, Tinku (2012) Polypyrrole/multiwalled carbon nanotubes-based biosensor for cholesterol estimation. Polymers for Advanced Technologies, 23 (7). pp. 1084-1091. ISSN 1042-7147

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Abstract

The nanocomposite electrode comprising of polypyrrole (PPY) and carboxy functionalized multiwalled carbon nanotubes (MWCNT) has been electrochemically fabricated onto indiumtinoxide (ITO) electrode using p-toluene sulfonic acid (PTS). Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) have been immobilized onto this PPY MWCNT/ITO nanocomposite electrode using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxy succinimide chemistry for estimation of esterified cholesterol. The ChEtChOx/PPYMWCNT/PTS/ITO bioelectrode has been characterized using Fourier transform infrared spectroscopy, electrochemical techniques, and scanning electron microscope. This ChEtChOx/PPYMWCNT/PTS/ITO nanobioelectrode has a response time of about 9?s, linearity of 4?x?10-4 to 6.5?x?10-3?M/l of cholesterol oleate concentration, Km of 0.02?mM, and thermal stability of upto 45 degrees C. This electrode exhibits improved biosensing characteristics compared with other total cholesterol electrodes reported in literature till date and can be used to estimate cholesterol in blood serum samples.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s Wiley
Subjects:	Polymer Science
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 11:03
Last Modified:	19 Aug 2026 11:03
URI:	https://npl.csircentral.net/id/eprint/3450

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