Joshi, Prachi and Chakraborti, Soumyananda and Ramirez-Vick, Jaime E. and Ansari, Z. A. and Shanker, Virendra and Chakrabarti, Pinak and Singh, Surinder P. (2012) The anticancer activity of chloroquine-gold nanoparticles against MCF-7 breast cancer cells. Colloids and Surfaces B-Biointerfaces, 95. pp. 195-200. ISSN 0927-7765

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Abstract

In the present study, 11-mercaptoundecanoic acid-modified gold nanoparticles (similar to 7 nm) were conjugated with chloroquine to explore their potential application in cancer therapeutics. The anticancer activity of chloroquine-gold nanoparticle conjugates (GNP-Chl) was demonstrated in MCF-7 breast cancer cells. The MCF-7 cells were treated with different concentrations of GNP-Chl conjugates, and the cell viability was assayed using trypan blue, resulting in an IC50 value of 30 +/- 5 mu g/mL. Flow cytometry analysis revealed that the major pathway of cell death was necrosis, which was mediated by autophagy. The drug release kinetics of GNP-Chl conjugates revealed the release of chloroquine at an acidic pH, which was quantitatively estimated using optical absorbance spectroscopy. The nature of stimuli-responsive drug release and the inhibition of cancer cell growth by GNP-Chl conjugates could pave the way for the design of combinatorial therapeutic agents, particularly nanomedicine, for the treatment of cancer.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s Elsevier
Subjects:	Biophysics Chemistry > Physical Chemistry Materials Science
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 10:44
Last Modified:	19 Aug 2026 10:44
URI:	https://npl.csircentral.net/id/eprint/3359

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