Singh, Jay and Srivastava, Manish and Roychoudhury, Appan and Lee, Dong Won and lee, Seung Hee and Malhotra, B. D. (2013) Bienzyme-Functionalized Monodispersed Biocompatible Cuprous Oxide/Chitosan Nanocomposite Platform for Biomedical Application. Journal of Physical Chemistry B, 117 (1). pp. 141-152. ISSN 1520-6106

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Abstract

The ultrafine monodispersed cuprous oxide (Ufm-Cu2O) nanoparticles have been successfully synthesized by a facile wet chemical method using poly-N-vinylpyrrolidone (PVP) as a capping agent. This colloidal solution of Ufm-Cu2O and chitosan (CS) is electrophoretically deposited (EPD) onto the indium tin-oxide (ITO) glass substrate. Thus synthesized nanocomposite has been characterized by X-ray powder diffraction (XRD, similar to 6 nm), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopic techniques. This novel biomedical nanocomposite platform has been explored to fabricate a cholesterol biosensor by immobilizing cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) onto Ufm-Cu2O-CS/ITO electrode surface. The seed germination tests of these biomaterials (Ufm-Cu2O-CS nanocomposite and ChOx-ChEtUfm-CuO2-CS nanobiocomposite), conducted using the disc diffusion method, reveal strong activity against the common pathogens and crops, indicating biocompatibility of the nanocomposite. Under optimized conditions, the linearity between the current response and the cholesterol concentration has been obtained in the range of 10-450 mg/dL, with detection limit of 15.9 mg/dLcm(-2) and a high sensitivity of 0.895 mu A/(mg/dLcm(-2)). The proposed biocompatible ChEt-ChOx/Ufm-Cu2O-CS/ITO bioelectrode shows fast response time (<5 s), good reproducibility, and long-term stability. This biocompatible biosensor has been used to determine the total cholesterol levels in human serum samples. Investigated antimicrobial activities of bienzyme-functionalized Ufm-Cu2O-CS nanocomposite are the potential platform for biomedical applications.

Item Type:	Article
Additional Information:	copyright for this article belongs to M/s American Chemical Society
Subjects:	Chemistry > Physical Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	19 Aug 2026 10:41
Last Modified:	19 Aug 2026 10:41
URI:	https://npl.csircentral.net/id/eprint/3331

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