Malhotra, Bansi Dhar and Prabhakar, Nirmal and Solanki, Pratima R.
(2008)
Conducting Polymer Based Nucleic Acid Sensor for Environment
Monitoring.
IEICE Transactions on Electronics , E91C (12).
pp. 1889-1893.
ISSN 1745-1353
Abstract
Nucleic acid sensor based on polyaniline has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClO-4) doped polyaniline (PANI) film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-(dimethylamino) propyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) chemistry. These dsCT-DNA-PANI/ITO and PANI/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, and Fourier-transform-infra-red (FTIR) measurements. This disposable dsCT-DNA-PANI/ITO bioelectrode is stable for about four months, can be used to detect arsenic trioxide (0.1ppm) in 30s.
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