Solanki, Pratima R. and Kaushik, Ajeet and Ansari, Anees A. and Sumana, G. and Malhotra, B. D. (2009) Horse radish peroxidase immobilized polyaniline for hydrogen peroxide sensor. Polymers for Advanced Technologies , 22 (6). 903-908. ISSN 1099-1581

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Abstract

Horse radish peroxidase (HRP) has been electrochemically entrapped into perchlorate (ClO4-) doped polyaniline (PANI) film deposited onto indium-tin-oxide (ITO) coated glass plate. This HRP-PANI-ClO4-/ITO bioelectrode characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), UV-Visible spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) techniques has been utilized for estimation of hydrogen peroxide (H2O2). This H2O2 sensor exhibits response time of 5 s, linearity from 3 to 136 mM, sensitivity as 0.5638 mu AmM(-1)cm(-2) with linear regression of 0.985. The value of the Michaelis-Menten constant (K-m) has been obtained as 1.984 mM.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Wiley.
Uncontrolled Keywords:	electrochemical entrapment; polyaniline; horse radish peroxidase; biosensor
Subjects:	Chemistry > Polymer Science
Divisions:	UNSPECIFIED
Depositing User:	Mr. Abhishek Yadav
Date Deposited:	03 Jan 2013 10:30
Last Modified:	03 Jan 2013 10:30
URI:	http://npl.csircentral.net/id/eprint/937

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