Singh, Shalini and Zack , Jyoti and Kumar, Dinesh and Srivastava , S. K. and -, Govind and Saluja , Daman and Khan, M. A. and Singh , P. K. (2010) DNA hybridization on silicon nanowires. Thin Solid Films, 519 (3). pp. 1151-1155. ISSN 0040-6090

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Nanowire-based detection strategies provide promising new routes to bioanalysis and indeed are attractive to conventional systems because of their small size, high surface-to-volume ratios, electronic, and optical properties. A sequence-specific detection of single-stranded oligonucleotides using silicon nanowires (SiNWs) is demonstrated. The surface of the SiNWs is functionalized with densely packed organic monolayer via hydrosilylation for covalent attachment. Subsequently, deoxyribonucleic acid (DNA) is immobilized to recognize the complementary target DNA. The biomolecular recognition properties of the nanowires are tested via hybridization with γP32 tagged complementary and non-complementary DNA oligonucleotides, showing good selectivity and reversibility. No significant non-specific binding to the incorrect sequences is observed. X-ray photoelectron spectroscopy, fluorescence imaging, and nanodrop techniques are used to characterize the modified SiNWs and covalent attachment with DNA. The results show that SiNWs are excellent substrates for the absorption, stabilization and detection of DNA sequences and could be used for DNA microarrays and micro fabricated SiNWs DNA sensors.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s. Elsevier B.V.
Uncontrolled Keywords: Silicon nanowire; DNA; FTIR; XPS
Subjects: Materials Science
Depositing User: Ms Neetu Chandra
Date Deposited: 04 Apr 2012 06:29
Last Modified: 04 Apr 2012 06:29

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