Das , Maumita and Dhand, Chetna and Sumana, Gajjala and Srivastava, A. K. and Nagarajan, R. and Nain, Lata and Iwamoto, M. and Manaka, Takaaki and Malhotra, B. D. (2010) Electrophoretic Fabrication of Chitosan-Zirconium-Oxide Nanobiocomposite Platform for Nucleic Acid Detection. Biomacromolecules, 12 (3). pp. 540-547. ISSN 1525-7797

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The present work describes electrophoretic fabrication of nanostructured chitosan−zirconium-oxide composite (CHIT−NanoZrO2) film (180 nm) onto indium−tin-oxide (ITO)-coated glass plate. This nanobiocomposite film has been explored as immobilization platform for probe DNA specific to M. Tuberculosis as model biomolecule to investigate its sensing characteristics. It is revealed that pH-responsive behavior of CHIT and its cationic skeleton is responsible for the movement of CHIT−NanoZrO2 colloids toward cathode during electrophoretic deposition. The FT-IR, SEM, TEM, and EDX techniques have been employed for the structural, morphological, and composition analysis of the fabricated electrodes. The morphological studies clearly reveal uniform inter-linking and dispersion of hexagonal nanograins of ZrO2 (30−50 nm) into the chitosan matrix, resulting in homogeneous nanobiocomposite formation. Electrochemical response measurements of DNA/CHIT−NanoZrO2/ITO bioelectrode, carried out using cyclic voltammetry and differential pulse voltammetry, reveal that this bioelectrode can specifically detect complementary target DNA up to 0.00078 μM with sensitivity of 6.38 × 10−6 AμM−1.

Item Type: Article
Uncontrolled Keywords: Copyright for this article belongs to M/s American Chemical Society.
Subjects: Biochemistry & Molecular Biology
Chemistry > Organic Chemistry
Polymer Science
Depositing User: Mr. Abhishek Yadav
Date Deposited: 10 Dec 2012 12:23
Last Modified: 10 Dec 2012 12:23
URI: http://npl.csircentral.net/id/eprint/885

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