Singh, Renu and Verma, Rachna and Kaushik, Ajeet and Sumana , Gajjala and Sood, Seema and Gupta , Rajinder K. and Malhotra, B.D. (2010) Chitosan–iron oxide nano-composite platform for mismatch-discriminating DNA hybridization for Neisseria gonorrhoeae detection causing sexually transmitted disease. Biosensors and Bioelectronics, 26 (6). pp. 2967-2974. ISSN 0956-5663

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Abstract

Electrochemically fabricated nano-composite film of chitosan (CH)–iron oxide (Fe3O4) has been used to detect gonorrhoea, a sexually transmitted disease (STD) via immobilization of biotinylated probe DNA (BDNA) using avidin–biotin coupling for rapid and specific (mismatch-discriminating) DNA hybridization. The presence of Fe3O4 nanoparticles (∼18 nm) increases the electro-active surface area of the nano-biocomposite that provides desirable environment for loading of DNA with better conformation leading to increased electron transfer kinetics between the medium and electrode. The differential pulse voltammetric (DPV) studies have been conducted using BDNA/avidin/CH–Fe3O4/ITO electrode owing to the reduction of the methylene blue (MB) indicator and investigate electron transfer between MB moieties and electrode for one and two-bases mismatch. This STD biosensor is found to have a detection limit (1 × 10−15 M) and a wide dynamic range (from 1 × 10−16 M to 1 × 10−6 M) using the complementary target DNA. In addition, the sensing system can be utilized to accurately discriminate complementary sequence from mismatch sequences.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Neisseria gonorrhoeae; Sexually transmitted disease; Chitosan-Fe(3)O(4) nanostructure; Methylene blue; Electrochemical sensor
Subjects:	Biophysics Biotechnology & Applied Microbiology Chemistry Chemistry > Analytical Chemistry Electrochemistry Nanoscience/ Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Mr. Abhishek Yadav
Date Deposited:	09 Nov 2012 11:33
Last Modified:	09 Nov 2012 11:33
URI:	http://npl.csircentral.net/id/eprint/813

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