Ansari, Anees A. and Singh, Renu and Sumana, G. and Malhotra, B. D. (2009) Sol–gel derived nano-structured zinc oxide film for sexually transmitted disease sensor. Analyst , 134 (5). pp. 997-1002. ISSN 1364-5528

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Abstract

A 20-mer thiolated oligonucleotide probe (th-ssDNA) specific to Neisseria gonorrhoeae immobilized onto a sol–gel derived nano-structured zinc oxide (ZnO) film dip-coated onto an indium-tin-oxide (ITO) glass substrate has been used for the fabrication of a DNA biosensor for sexually transmitted disease (gonorrhoea) detection using hybridization technique. The results of characterization studies carried out on this th-ssDNA–ZnO/ITO bioelectrode using X-ray diffraction, UV-Visible, Fourier-transform infrared, scanning electron microscopy and electrochemical techniques reveal the linearity as 0.000524 fmol–0.524 nmol, with a detection limit of 0.000704 fmol within 60 s.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Royal Society of Chemistry.
Subjects:	Chemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	04 Oct 2012 06:27
Last Modified:	04 Oct 2012 06:27
URI:	http://npl.csircentral.net/id/eprint/642

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