Tiwari, Ashutosh (2009) A Novel Nanocomposite Matrix Based on Silylated Chitosan and Multiwall Carban Nanotubes for the Immobilization of Urease. Journal of Inorganic and Organometallic Polymers and Materials , 19 (3). pp. 361-366. ISSN 1574-1451

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Abstract

A nanocomposite matrix made up of silylated chitosan and multiwall carbon nanotubes (CHIT–SiO2–MWCNTs) was fabricated to investigate the immobilization of urease (Urs). Urs enzyme was covalently immobilized with the CHIT–SiO2–MWCNTs matrix using glutaraldehyde as a linker. The resulting Urs/CHIT–SiO2–MWCNTs biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry (CV) taking CHIT–SiO2–MWNTs as a reference. The influence of various parameters on Urs enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis–Menten constant and apparent activities for the Urs enzyme were calculated to be 0.51 mM and 89.02 mg/cm2, respectively; indicating CHIT–SiO2–MWCNTs nanocomposite matrix has a high affinity to immobilize Urs enzyme.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Springer Verlag.
Uncontrolled Keywords:	Chitosan; Multiwall carbon nanotubes; Nanocomposite matrix; Covalent immobilization; Urease
Subjects:	Polymer Science
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	21 Sep 2012 06:45
Last Modified:	21 Sep 2012 07:11
URI:	http://npl.csircentral.net/id/eprint/587

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