Ali, Azahar and Ansari, Anees A. and Kaushik, Ajeet and Solanki, Pratima R. and Barik, A. and Pandey, M. K. and Malhotra, B. D. (2009) Nanostructured zinc oxide film for urea sensor. Materials Letters , 63 (28). pp. 2473-2475. ISSN 0167-577X

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Abstract

Nanostructured zinc oxide (Nano-ZnO) film has been electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate to co-immobilized urease (Urs) and glutamate dehydrogenase (GLDH) for urea detection. The observed reflection planes corresponding to wurtzite ZnO nanoparticles (~25 nm) in XRD diffraction pattern and UV-visible absorption band at 338 nm reveal the formation of Nano-ZnO. Urs-GLDH/Nano-ZnO/ITO bioelectrode shows high sensitivity for urea detection within 10–80 mg/dL and limit of detection as 13.5 mg/dL with regression coefficient as 0.994 and Michaelis–Menten constant (Km, 6.1 mg/dL) indicating good affinity of Urs-GLDH to urea.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Nanostructured ZnO; Nanomaterials; Sensor; Electrochemical deposition; Enzyme
Subjects:	Materials Science Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	19 Sep 2012 12:51
Last Modified:	19 Sep 2012 12:51
URI:	http://npl.csircentral.net/id/eprint/578

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