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Abstract

Biotinylated heparin has been immobilized onto self-assembled monolayer of 4-aminothiophenol using avidin–biotin specific binding. The modified electrodes have been characterized using surface plasmon resonance technique (SPR), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and contact angle (CA) measurements. The interaction of immobilized biotinylated heparin with low density lipoprotein (LDL) has been studied using surface plasmon resonance technique. The biotinylated heparin modified electrode can be used to detect LDL in the range of 20 to 100 mg/dl with the sensitivity of 513.3 m°/μM.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords:	Self-assembled monolayer; Surface plasmon resonance; Heparin; Low density lipoprotein
Subjects:	Materials Science Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	10 Sep 2012 07:36
Last Modified:	10 Sep 2012 07:36
URI:	http://npl.csircentral.net/id/eprint/556

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