Matharu, Zimple and Bandodkar, Amay Jairaj and Sumana, G. and Solanki, Pratima R. and Ekanayake, E. M. I. Mala and Kaneto, Keiichi and Gupta, Vinay and Malhotra, B. D. (2009) Low Density Lipoprotein Detection Based on Antibody Immobilized Self-Assembled Monolayer: Investigations of Kinetic and Thermodynamic Properties. Journal of Physical Chemistry B , 113 (43). pp. 14405-14412. ISSN 1520-5207

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Abstract

Human plasma low density lipoprotein (LDL) immunosensor based on surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) was fabricated by immobilizing antiapolipoprotein B (AAB) onto self-assembled monolayer (SAM) of 4-aminothiophenol (ATP). The AAB/ATP/Au immunosensor can detect LDL up to 0.252 μM (84 mg/dL) and 0.360 μM (120 mg/dL) with QCM and SPR, respectively. The SPR and QCM measurements were further utilized to study the reaction kinetics of the AAB-LDL interaction. The adsorption process involved was explored using Langmuir adsorption isotherm and Freundlich adsorption models. The thermodynamic parameters such as change in Gibb’s free energy (ΔGads), change in enthalpy (ΔHads), and change in entropy (ΔSads) determined at 283, 298, and 308 K revealed that the AAB-LDL interaction is endothermic in nature and is governed by entropy. Kinetic, thermodynamic, and sticking probability studies disclosed that desorption of the water molecules from the active sites of AAB and LDL plays a key role in the interaction process and increase in temperature favors binding of LDL with the AAB/ATP/Au immunosensor. Thus, the studies were utilized to unravel the most important subprocess involved in the adsorption of LDL onto AAB-modified ATP/Au surface that may help in the fabrication of LDL immunosensors with better efficiency.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s American Chemical Society.
Subjects:	Biochemistry & Molecular Biology Chemistry Medical Laboratory Technology Thermodynamics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	10 Sep 2012 07:27
Last Modified:	10 Sep 2012 07:27
URI:	http://npl.csircentral.net/id/eprint/555

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