Matharu, Zimple and Pandey, Pratibha and Pandey, M. K. and Gupta, Vinay and Malhotra, B. D. (2009) Functionalized Gold Nanoparticles – Octadecylamine Hybrid Langmuir-Blodgett Film for Enzyme Sensor. Electroanalysis , 21 (14). pp. 1587-1596. ISSN 1521-4109

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Abstract

Mediator free enzyme sensor has been fabricated by covalently immobilizing cholesterol oxidase (ChOx) onto 11-mercaptoundecanoic acid functionalized gold nanoparticles (MUDA-AuNPs) – octadecylamine (ODA) hybrid Langmuir–Blodgett film. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) studies reveal that MUDA-AuNP/ODA LB film has good affinity for ChOx and provides favorable microenvironment for direct electron transfer between enzyme and electrode. Interference free estimation of cholesterol has been realized at 0.3 V with linear range from 25 to 500 mg/dL, detection limit of 23.38 mg/dL, sensitivity of 1.085 μA mM−1 and response time of 20 s at pH 7.0.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Wiley-VCH Verlag Berlin
Uncontrolled Keywords:	Functionalized gold nanoparticles; Langmuir-Blodgett film; Cholesterol oxidase; Cholesterol sensor; Biosensors; Enzymes
Subjects:	Chemistry Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	31 Aug 2012 07:15
Last Modified:	31 Aug 2012 07:15
URI:	http://npl.csircentral.net/id/eprint/513

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