Ansari, Anees A. and Kaushik, Ajeet and Solanki, Pratima R. and Malhotra , B. D. (2008) Electrochemical Cholesterol Sensor Based on Tin Oxide-Chitosan Nanobiocomposite Film. Electroanalysis , 21 (8). pp. 965-972. ISSN 1521-4109

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Abstract

A chitosan (CS)-tin oxide (SnO2) nanobiocomposite film has been deposited onto an indium-tin-oxide glass plate to immobilize cholesterol oxidase (ChOx) for cholesterol detection. The value of the Michaelis–Menten constant (Km) obtained as 3.8 mM for ChOx/CS-SnO2/ITO is lower (8 mM) than that of a ChOx/CS/ITO bioelectrode revealing enhancement in affinity and/or activity of ChOx towards cholesterol and also revealing strong binding of ChOx onto CS-SnO2/ITO electrode. This ChOx/CS-SnO2/ITO cholesterol sensor retains 95% of enzyme activity after 4–6 weeks at 4 °C with response time of 5 s, sensitivity of 34.7 μA/mg dL−1 cm2 and detection limit of 5 mg/dL.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Wiley-VCH Verlag Berlin
Uncontrolled Keywords:	Chitosan; SnO2 nanoparticles; Cholesterol biosensor; Nanobiocomposite
Subjects:	Chemistry Electrochemistry
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	27 Aug 2012 08:36
Last Modified:	27 Aug 2012 08:36
URI:	http://npl.csircentral.net/id/eprint/478

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