Bhardwaj, Hema and Marquette, Christophe A. and Dutta, Priyanka and -, Rajesh and Sumana, Gajjala (2020) Integrated graphene quantum dot decorated functionalized nanosheet biosensor for mycotoxin detection. Analytical and Bioanalytical Chemistry , 412 (25). pp. 7029-7041. ISSN 1618-2642

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Decoration of graphene quantum dots (GQDs) on molybdenum disulfide (MoS2) nanosheets serves as an active electrode material which enhances the electrochemical performance of the analyte detection system. Herein, ionic surfactant cetyltrimethylammonium bromide (CTAB)-exfoliated MoS2 nanosheets decorated with GQD material are used to construct an electrochemical biosensor for aflatoxin B1 (AFB1) detection. An antibody of AFB1 (aAFB1) was immobilized on the electrophoretically deposited MoS2@GQDs film on the indium tin oxide (ITO)-coated glass surface using a crosslinker for the fabrication of the biosensor. The immunosensing study investigated by the electrochemical method revealed a signal response in the range of 0.1 to 3.0 ng/mL AFB1 concentration with a detection limit of 0.09 ng/mL. Also, electrochemical parameters such as diffusion coefficient and heterogeneous electron transfer (HET) were calculated and found to be 1.67 x 10(-5) cm(2)/s and 2 x 10(-5)cm/s, respectively. The effective conjugation of MoS2@GQDs that provides abundant exposed edge sites, large surface area, improved electrical conductivity, and electrocatalytic activity has led to an excellent biosensing performance with enhanced electrochemical parameters. Validation of the fabricated immunosensor was performed in a spiked maize sample, and a good percentage of recoveries within an acceptable range were obtained (80.2 to 98.3%).

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Springer.
Subjects: Chemistry > Analytical Chemistry
Biochemical Research Methods
Depositing User: Mr. Yogesh Joshi
Date Deposited: 25 Mar 2022 11:36
Last Modified: 25 Mar 2022 11:36

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