Verma, Shilpi and Arya, Priyanshu and Singh, Anu and Kaswan, Jyoti and Shukla, Ajay and Kushwaha, Hemant R. and Gupta, Shalini and Singh, Surinder P. (2020) ZnO-rGO nanocomposite based bioelectrode for sensitive and ultrafast detection of dopamine in human serum. Biosensors and Bioelectronics, 165. pp. 112347-112354. ISSN 0956-5663

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We present a tyrosinase-conjugated zinc oxide-reduced graphene oxide (Tyr/ZnO-rGO) nanocomposite system as a biosensing test-bed for rapid and sensitive detection of dopamine (DA). The bioelectrodes (Tyr/ZnO-rGO/ITO) were designed by covalently immobilizing tyrosinase enzyme on spin-coated films of ZnO-rGO nanocomposite prepared via self-assembly approach. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) showed fast electron transfer kinetics of ZnO-rGO/ITO electrode. The response studies of the Tyr/ZnO-rGO/ITO bioelectrode revealed ultrafast (0.34 +/- 0.09 s) detection of DA in a wide linear dynamic range of 0.1-1500 pM. The significant performance of the biosensor in terms of low detection limit (8.75 +/- 0.64 pM) and high sensitivity (39.56 +/- 0.41 mu A nM(-1)) values is attributed to the fast and unhindered electron transfer mechanism of ZnO-rGO matrix having low electrochemical band gap. The nanoplatform exhibited high selectivity toward DA in human sera, and remained stable up to 3 months at 4 degrees C, representing its suitability for clinical applications.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Elsevier.
Subjects: Biophysics
Biotechnology & Applied Microbiology
Chemistry > Analytical Chemistry
Nanoscience/ Nanotechnology
Depositing User: Mr. Yogesh Joshi
Date Deposited: 09 Nov 2021 11:18
Last Modified: 09 Nov 2021 11:18

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