Jaiswal, Nandita and Pandey, Chandra Mouli and Soni, Amrita and Tiwari, Ida and Rosillo-Lopez, Martin and Salzmann, Christoph G. and Malhotra, Bansi Dhar and Sumana, Gajjala (2018) Electrochemical genosensor based on carboxylated graphene for detection of water-borne pathogen. Sensors and Actuators B: Chemical, 275. pp. 312-321. ISSN 0925-4005

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This work reports the application of newly synthesized carboxylated graphene nanoflakes (Cx-Gnfs). The Cx-Gnfs were synthesized by wet chemical method in sulfuric acid/nitric acid mixture and was further electrophoretically deposited on indium fin oxide (ITO) coated glass substrates using Mg(2+ )ions which provides an overall charge to the materials for deposition onto the anode. The materials were characterized using SEM, TEM, contact angle, UV-vis spectroscopy, FT-IR, XRD and electrochemically characterized by cyclic voltammetry, chronocoulometry and electrochemical impedance spectroscopy. The sensitive quantitative determination of nucleic acid Escherichia coli O157: H7 (E. coil) has been achieved using Cx-Gnfs and r-GO as the sensing layer using electrochemical impedance spectroscopy. The electrochemical results reveal that the Cx-Gnfs based genosensor exhibits a linear response to complementary DNA (10(-6) M to 10(-17) M) with a detection limit of 1 x 10(-17) M while the rGO based genosensor shows a detection limit of 1 x 10(-15) M. Under optimal conditions, this Cx-Gnfs based genosensor was found to retain about 85% of its initial activity after being used for 6 times.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Elsevier.
Subjects: Chemistry > Analytical Chemistry
Instruments/ Instrumentation
Depositing User: Mr. Yogesh Joshi
Date Deposited: 03 Apr 2019 11:07
Last Modified: 03 Apr 2019 11:07
URI: http://npl.csircentral.net/id/eprint/4041

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