Bhardwaj, Hema and Singh, Chandan and Kotnala, R. K. and Sumana, Gajjala (2018) Graphene quantum dots-based nano-biointerface platform for food toxin detection. Analytical and Bioanalytical Chemistry , 410 (28). pp. 7313-7323. ISSN 1618-2642

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Due to the similar electrochemical properties to graphene oxide (GO), graphene quantum dots (GQDs) are considered as a highly potential candidate for designing an electrochemical biosensor. In this report, GQDs were synthesized having anaverage diameter of 7nm and utilized for the fabrication of anelectrochemical immunosensor for the detection of food toxin, aflatoxin B-1 (AFB(1)). An electrophoretic deposition technique was utilized to deposit the chemically synthesized GQDs onto indium tin oxide (ITO)-coated glass substrate. Further, the monoclonal antibodies of AFB(1) were covalently immobilized onto deposited electrode GQDs/ITO using EDC-NHS as a crosslinker. The structural and morphological studies of GQDs and conjugated anti-AFB(1) with GQDs have been investigated using UV-visible spectroscopy, photoluminescence spectroscopy, Raman spectroscopy, transmission electron microscopy, scanning electron microscopy techniques, etc. The electrochemical impedance spectroscopy and cyclic voltammetry measurements were carried out for electrical characterization and biosensing studies. This simple monodisperse GQDs-based platform yields heterogeneous electron transfer (97.63x10(-5) cm s(-1)), the time constant (0.005s) resulting in improved biosensing performance. The electrochemical immunosensor shows high sensitivity 213.88 (ngmL(-1))(-1)cm(-2). The limit of detection for standard samples and contaminated maize samples wasfound to be 0.03ngmL(-1) and 0.05ngg(-1), respectively, which is lower than the maximum acceptable limit according to the European Union. This result indicates its potential application for aflatoxin B-1 detection in food contents.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Elsevier.
Subjects: Biochemistry & Molecular Biology
Chemistry > Analytical Chemistry
Depositing User: Mr. Yogesh Joshi
Date Deposited: 21 Nov 2019 07:17
Last Modified: 21 Nov 2019 07:17

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