Singh, Chandan and Ali, Md. Azahar and Reddy, Venu and Singh, Dinesh and Kim, Cheol Gi and Sumana, G. and Malhotra, B. D. (2018) Biofunctionalized graphene oxide wrapped carbon nanotubes enabled microfluidic immunochip for bacterial cells detection. Sensors and Actuators B: Chemical, 255 (3). pp. 2495-2503. ISSN 0925-4005

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Abstract

A sensitive and selective microfluidic immunochip was fabricated for detection ofSalmonella typhimurium (S. typhimurium) bacterial cells. In this sensor, graphene oxide (GO) nano sheets wrapped carboxylated multiwalled carbon nanotubes (cMWCNTs) composite acted as a transducer material. The colloidal solution of GO-cMWCNTs composite was selectively deposited onto patterned indium tin oxide (ITO) electrode and sealed with polydimethylsiloxane (PDMS) micro channels. The S. typhimurium antibodies (StAb) were in situ biofunctionalized followed by EDC-NHS covalent chemistry via amidation reaction. The presence of abundant functional groups at the GO-cMWCNTs composite improved the loading of antibodies (StAb) against S. typhimurium leading to improved biosensing characteristics. Wrapping of cMWCNTs with GO resulted in superior electron transfer behavior enhancing the sensitivity (162.47 mu A/CFU-1/mLcm(-2)) almost two folds as compared to that based on GO (89.16 mu A/CFU-1/mLcm(-2)) sheets for bacterial cells detection. Besides this, GO wrapped cMWCNTs integrated microfluidics biosensor offered low detection limit as 0.376 CFU/mL and negligible interference due to presence of Escherichia coli (E. coli ( 0157:H7).

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier.
Subjects:	Chemistry > Analytical Chemistry Electrochemistry Instruments/ Instrumentation
Divisions:	UNSPECIFIED
Depositing User:	Mr. Yogesh Joshi
Date Deposited:	17 Oct 2019 07:59
Last Modified:	17 Oct 2019 07:59
URI:	http://npl.csircentral.net/id/eprint/3866

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