Srivastava, Shubhda and Senguttuvan, Thanikachalam Devarajan and Gupta, Bipin Kumar (2018) Highly efficient fluorescence quenching with chemically exfoliated reduced graphene oxide. Journal of Vacuum Science and Technology B Microelectronics and Nanometer Structures, 36 (4). 04G104-04G108. ISSN 1071-1023

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Abstract

Fluorescence quenching is a valuable tool to study many macromolecular assemblies. It is well-known that graphitic systems such as the reduced graphene oxide (rGO) can strongly quench the emission of dye molecules through energy transfer. In this work, the authors demonstrate comparative study of fluorescence quenching between different graphitic system such as rGO, graphene oxide (GO), and graphite, which is not reported so far. As compared to graphite and GO sheets, rGO shows more quenching capability because of more surface area and efficient p-p stacking. The fluorescence quenching has been performed using Rhodamine B dye. The scanning electron microscopic micrographs revealed that Rhodamine B dye is uniformly absorbed on rGO. The fluorescence quenching has been done with the fixed concentration of Rhodamine organic dye with all three forms of carbon. The spectroscopic results exhibit that the chemically exfoliated rGO was almost 16 times better than graphite and GO. The obtained results suggest that rGO can be used for better quenching capability in biosensing applications.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s American Vacuum Society.
Subjects: Engineering > Electronics and Electrical Engineering
Applied Physics/Condensed Matter
Nanoscience/ Nanotechnology
Divisions: UNSPECIFIED
Depositing User: Users 27 not found.
Date Deposited: 17 Dec 2019 08:23
Last Modified: 17 Dec 2019 08:23
URI: http://npl.csircentral.net/id/eprint/3794

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