Sinha, Sujata and Dhakate, Sanjay R. and Kumar, Pankaj and Mathur, R. B. and Tripathi, Pushplata and Chand, Subhash (2012) Electrospun polyacrylonitrile nanofibrous membranes for chitosanase immobilization and its application in selective production of chitooligosaccharides. Bioresource Technology, 115. pp. 152-157. ISSN 0960-8524

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Polyacrylonitrile nanofibrous membranes (PANNFM) were prepared by electrospinning from 10 wt.% of PAN solution and its surface was modified by amidination reaction. A new chitosan degrading enzyme from Aspergillus sp. was covalently immobilized on PANNFM. Immobilization efficiency of 80% was achieved by activating PANNFM surface for 30 min followed by 2 h treatment with enzyme solution. The optimum temperature and pH for immobilized enzyme were 50 degrees C and 5.8, respectively. The immobilized chitosanase retained >70% activity after ten repeated batch reaction and could be stored up to 60 days at 4 degrees C with minor loss in activity. Chitosan hydrolysis using different substrates were studied using immobilized chitosanase in batch conditions. Continuous selective production of chitooligosaccharides (dimer to hexamer) by changing the temperature was achieved by PANNFM-chitosanase.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Elsevier.
Subjects: Biotechnology & Applied Microbiology
Energy Fuels
Agricultural Engineering
Depositing User: Users 27 not found.
Date Deposited: 04 Mar 2020 11:31
Last Modified: 04 Mar 2020 11:31

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