Singh, K. and Singh, B. P. and Chauhan, Ruchika and Basu, T. (2012) Fabrication of amperometric bienzymatic glucose biosensor based on MWCNT tube and polypyrrole multilayered nanocomposite. Journal of Applied Polymer Science, 125. E235-E246. ISSN 0021-8995

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Abstract

A novel amperometric glucose biosensor based on bienzyme system such as glucose oxidase (GOx) and horseradish peroxidase (HRP) was developed based on the carboxy modified multiwalled carbon nanotubes (MWCNT) and polypyrrole (PPY) nanocomposite film, electrochemically deposited onto indium-tin-oxide (ITO) electrode using layer by layer deposition technique. The glucose oxidase (GOx) and horseradish peroxidase (HRP) have been coimmobilized onto the MWCNT/PPY/ITO nanocomposite electrode using gluteraldehyde as a crosslinking agent. The bienzymatic nanobioelectrodes GOx-HRP/MWCNT/PPY/ITO have exhibited 3 times higher sensitivity than mono enzymatic bioelectrode (GOx-HRP/MWCNT/PPY/ITO). Minimum interferences have been observed from ascorbic acid, uric acid, sodium pyruvate, and sodium ascorbate for both single and bienzyme systems. It is inferred that bienzyme-based nanobioelectrodes offer wider linearity, higher sensitivity, correlation coefficient, shelf life, and accuracy for testing of blood serum samples than mono enzyme system. Mechanism of the overall biochemical reaction has been proposed to illustrate the enhanced bio-sensing performance of the bienzyme system.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Wiley.
Subjects:	Polymer Science
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	12 Mar 2020 09:40
Last Modified:	12 Mar 2020 09:40
URI:	http://npl.csircentral.net/id/eprint/3570

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