Solanki, Pratima R. and Ali, Md. Azahar and Agrawal, Ved V. and Srivastava, A. K. and Kotnala, R. K. and Malhotra, B. D. (2013) Highly sensitive biofunctionalized nickel oxide nanowires for nanobiosensing applications. RSC Advances , 3 (36). pp. 16060-16067. ISSN 2046-2069

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We report results of the studies relating to the fabrication of nickel oxide nanowires (NwNiOs) with an enhanced aspect ratio of similar to 100 for biosensing applications. Anti Vibrio cholerae monoclonal antibodies were used to functionalize the nickel oxide nanowire (20-80 nm) surfaces fabricated on indium tin oxide coated glass plate for Vibrio cholerae detection. The results of the impedance response studies conducted using this immunoelectrode as a function of the Vibrio cholerae concentration revealed a detection range of 37-350 ng ml(-1) and a low detection limit of 0.553 ng ml(-1) using the 3 sigma(b)/m criteria. The high sensitivity (11.12 Omega (ng ml(-1))(-1) cm(-2)) of this fabricated sensor is attributed to the excellent electronic properties of NiO nanowires which facilitate the efficient transfer of electrons between the electrode and the antibody molecules through electron channeling effects. Besides this, the nanostructured NiO nanowire based immunosensor exhibits interesting supercapacitive behaviour towards the detection of CT. This immunosensor showed values of association constant (K-a) of 4.5 x 10(7) ng ml(-1) and dissociation constant (K-d) of 2.22 x 10(-6) ng ml(-1).

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Royal Society of Chemistry.
Subjects: Chemistry
Depositing User: Users 27 not found.
Date Deposited: 09 Nov 2021 09:53
Last Modified: 09 Nov 2021 09:53

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