Patel, Manoj K. and Ali, Md. Azahar and Agrawal, Ved V. and Ansari, Z. A. and Ansari, S. G. and Malhotra, B. D. (2013) Nanostructured magnesium oxide biosensing platform for cholera detection. Applied Physics Letters, 102 (14). 144106-1-144106-5. ISSN 0003-6951

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Abstract

We report fabrication of highly crystalline nanostructured magnesium oxide (NanoMgO, size >30 nm) film electrophoretically deposited onto indium-tin-oxide (ITO) glass substrate for Vibrio cholerae detection. The single stranded deoxyribonucleic acid (ssDNA) probe, consisting of 23 bases (O1 gene sequence) immobilized onto NanoMgO/ITO electrode surface, has been characterized using electrochemical, Fourier Transform-Infra Red, and UltraViolet-visible spectroscopic techniques. The hybridization studies of ssDNA/NanoMgO/ITO bioelectrode with fragmented target DNA conducted using differential pulse voltammetry reveal sensitivity as 16.80 nA/ng/cm(2), response time of 3 s, linearity as 100-500 ng/mu L, and stability of about 120 days.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s American Institute of Physics.
Subjects:	Applied Physics/Condensed Matter
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	09 Jul 2020 10:00
Last Modified:	09 Jul 2020 10:00
URI:	http://npl.csircentral.net/id/eprint/3112

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