Patel, Manoj K. and Singh, Jay and Singh, Manish K. and Agrawal, Ved Varun and Ansari, S. G. and Malhotra, B. D. (2013) Tin Oxide Quantum Dot Based DNA Sensor for Pathogen Detection. Journal of Nanoscience and Nanotechnology, 13 (3). pp. 1671-1678. ISSN 1533-4880

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Abstract

We report the application of nano crystalline tin oxide quantum dots (SnO2-QDs) for electrochemical detection of Vibrio cholerae based on DNA hybridization technique. SnO2-QDs (similar to 1-5 nm) have been synthesized by laser ablation technique in liquid (LAL) and electrophoretically deposited onto hydrolyzed surface of indium tin oxide (ITO) coated glass electrode. A single stranded oligonucleotide probe (23 bases) have been designed form the virulent gene sequence of V cholerae and has been immobilized onto SnO2-QDs/ITO surface for the fabrication of ssDNA/SnO2-QDs/ITO bioelectrode and these bioelectrode have been further used for DNA hybridization (dsDNA/SnO(2-)QDs/ITO). The electrochemical response studies have been carried out with different concentration genomic DNA (100-500 ng/mu L), which indicated that SnO2 provides an effective surface to bind with the phosphate group of DNA, thus resulting in an enhanced electron transport. The hybridized electrode exhibits linear response with regression coefficient (R) 0.974, high sensitivity 35.20 nA/ng/cm(2), low detection limit (31.5 ng/mu L), faster response time (3 s) and high stability of 0-120 days when stored under refrigerated conditions.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s American Scientific Publishers.
Subjects:	Chemistry Materials Science Applied Physics/Condensed Matter Nanoscience/ Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	28 May 2020 11:12
Last Modified:	28 May 2020 11:12
URI:	http://npl.csircentral.net/id/eprint/2987

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