Dhand , Chetna and Solanki, Pratima R. and Datta, Monika and Malhotra, B. D. (2010) Polyaniline/Single-Walled Carbon Nanotubes Composite Based Triglyceride Biosensor. Electroanalysis , 22 (22). pp. 2683-2693. ISSN 1521-4109

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Nanocomposite film comprising of polyaniline (PANI) and single walled carbon nanotubes (SWCNT) has been fabricated onto indium-tin-oxide (ITO) coated glass plate using electrophoretic technique. Co-immobilization of glycerol dehydrogenase (GDH) and lipase (LIP) has been done via N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry to explore its application for triglyceride (tributyrin) sensing. Response studies have been done using linear sweep voltammetry revealing that LIP-GDH/PANI-SWCNT-TB/ITO bioelectrode can detect tributyrin in the range of 50 to 400 mg dL−1 with low Michaelis–Menten constant of 1.138 mM, improved response time of 12 s, high sensitivity as 4.28×10−4 mA mg−1 dL and storage stability of about 13 weeks.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Wiley-VCH Verlag Berlin.
Uncontrolled Keywords: Biosensors; Carbon nanotubes; Electrophoretic deposition; * Polyaniline; Triglyceride; Nanotubes
Subjects: Chemistry
Depositing User: Ms Neetu Chandra
Date Deposited: 20 Jun 2012 06:36
Last Modified: 20 Jun 2012 06:36
URI: http://npl.csircentral.net/id/eprint/280

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