Singh, Renu and Dhand, Chetna and Sumana, Gajjala and Verma, Rachna and Sood, Seema and Gupta, Rajinder Kumar and Malhotra, Bansi Dhar (2010) Polyaniline/carbon nanotubes platform for sexually transmitted disease detection. Journal of Molecular Recognition , 23 (5). pp. 472-479. ISSN 1099-1352

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Abstract

Polyaniline/carbon nanotubes composite (PANI-CNT) electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate has been utilized for Neisseria gonorrhoeae detection by immobilizing 5′-amino-labeled Neisseria gonorrhoeae probe (aDNA) using glutaraldehyde as a cross-linker. PANI-CNT/ITO and aDNA-Glu-PANI-CNT/ITO electrodes have been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This bioelectrode can be used to detect N. gonorrhoeae using methylene blue (MB) as redox indicator with response time of 60 s and stability of about 75 days when stored under refrigerated conditions. DPV studies reveal that this bioelectrode can detect complementary DNA concentration from 1 × 10−6 M to 1 × 10−17 M with detection limit of 1.2 × 10−17 M. Further, this bioelectrode (aDNA-Glu-PANI-CNT/ITO) exhibits specificity toward N. gonorrhoeae species and shows negative response with non-Neisseria gonorrhoeaeNeisseria species (NgNS) and other gram negative bacteria (GNB).

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s John Wiley and Sons
Uncontrolled Keywords:	polyaniline; carbon nanotubes; sexually transmitted disease; Neisseria gonorrhoeae; DNA biosensor;
Subjects:	Biochemistry & Molecular Biology Biophysics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	18 Jun 2012 11:23
Last Modified:	18 Jun 2012 11:23
URI:	http://npl.csircentral.net/id/eprint/279

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