Abdul, Barik and Solanki, Pratima R. and Kaushik, Ajeet and Ali, Azahar and Pandey, M. K. and Kim, C. G. and Malhotra, B. D. (2010) Polyaniline–Carboxymethyl Cellulose Nanocomposite for Cholesterol Detection. Journal of Nanoscience and Nanotechnology , 10 (10). pp. 6479-6488. ISSN 1533-4899

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Abstract

Cholesterol oxidase (ChOx) has been covalently immobilized onto polyaniline-carboxymethyl cellulose (PANI-CMC) nanocomposite film deposited onto indium-tin-oxide (ITO) coated glass plate using glutaraldehyde as a cross-linker. Fourier transform infrared (FTIR) spectroscopic and electrochemical studies have been used to characterize the PANI-CMC/ITO nanocomposite electrode and ChOx/PANI-CMC/ITO bioelectrode. Scanning electron microscopy (SEM) studies reveal the formation of PANI-CMC nanocomposite fibers of size ∼150 nm in diameter. The ChOx/PANI-CMC/ITO bioelectrode exhibits linearity as 0.5-22 mM, detection limit as 1.31 mM, sensitivity as 0.14 mA/mM cm2, response time as 10 s and shelf-life of about 10 weeks when bioelectrode is stored at 4 °C. The low value of Michaelis-Menten constant (Km) obtained as 2.71 mM reveals high affinity of immobilized ChOx for PANI-CMC/ITO nanocomposite electrode.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s American Scientific Publishers
Subjects:	Biochemistry & Molecular Biology Chemistry Materials Science Mathematics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	18 Jun 2012 11:13
Last Modified:	18 Jun 2012 11:13
URI:	http://npl.csircentral.net/id/eprint/278

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