Ali, Md. Azahar and Singh, Chandan and Srivastava, Saurabh and Admane, Prasad and Agrawal, Ved V. and Sumana, Gajjala and John, Renu and Panda, Amulya and Dong, Liang and Malhotra, Bansi D. (2017) Graphene oxide-metal nanocomposites for cancer biomarker detection. RSC Advances , 7. pp. 35982-35991. ISSN 2046-2069

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We report a universal protocol for the in situ bioinspired green synthesis of metal (Pd, Pt, Ag and Au) nanoparticles (mNPs) on simultaneously reduced graphene oxide (rGO) sheets using a black pepper extract (BPE) for quantification and kinetic analysis of epidermal growth factor receptor (ErbB2) for application to breast cancer diagnostics. The phytochemicals present in BPE were found to assist the reduction, stabilization, and surface addition of mNPs onto rGO sheets. The antioxidants present in the BPE assist the formation of a "green corona" on the surface of derived G-mNPs@rGO (G-Pd@rGO, GPt@ rGO, G-Ag@rGO, and G-Au@rGO) sheets as corroborated by DLS and FT-IR experiments. These bioinspired derivatives provided biocompatible surfaces, resulting in higher cell viability compared to that of chemically derived composites, C-mNPs@rGO (C-Pd@rGO, C-Pt@rGO, C-Ag@rGO, and C-Au@rGO). Toxicity was found to be drastically reduced for green-corona assisted derivatives of GO due to strong antioxidant properties of the phytochemicals present in BPE. In addition, a comparative analytical study of the quantification and kinetics of breast cancer biomarkers using both G-mNPs@rGO and CmNPs@ rGO nanocomposites was carried out. The G-mNPs@rGO nanosheets can be employed for ultrasensitive detection of ErbB2 concentration from 1.0 fM to 0.5 mu M.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Royal Society of Chemistry.
Subjects: Chemistry
Chemistry > Analytical Chemistry
Biochemical Research Methods
Depositing User: Users 27 not found.
Date Deposited: 10 Oct 2018 08:19
Last Modified: 10 Oct 2018 08:19

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