Cao, Shunsheng and Mishra, Rajeev and Pilla, Srikanth and Tripathi, Swapnil and Pandey, Manoj K. and Shah, Gopit and Mishra, Ajay K. and Prabaharan, Mani and Mishra, Shivani B. and Xin, Jin and Pandey, R. R. and Wu, Weiwei and Pandey, Avinash C. and Tiwari, Ashutosh (2010) Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection. Carbohydrate Polymers , 82 (1). pp. 189-194. ISSN 0144-8617

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A 20-mer single-stranded oligodeoxyribonucleotide (ssODNs) was covalently probed onto the nanocomposite electrode, made up of an indium-tin oxide (ITO) glass surface coated with chitosan (CHIT), which is bonded with carboxyl functionalized thiol capped gold nanoparticles (gold-mercaptopropionic acid, Au-MPA NPs). The prepared CHIT/Au-MPA/ITO and the ssODNs/CHIT/Au-MPA/ITO electrodes were characterized with FTIR spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). TEM micrograph of the composite electrode showed that Au-MPA NPs had the diameter ranging from 4 to 16 nm. The ssODNs/CHIT/Au-MPA/ITO electrode showed a linear increase in the amperometric current with an increasing concentration of the single-stranded target complementary ODNs (cODNs) within the range of 0.03–325 fM. The ssODNs/CHIT/Au-MPA/ITO electrode exhibited a sensitivity of 0.572 μA/fM with a response time of 12 s. The ssODNs/CHIT/Au-MPA/ITO electrode had a shelf life of ∼182 days, when stored at 4 °C.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Elsevier B.V.
Uncontrolled Keywords: DNA biosensor; Sequence-specific detection; Chitosan; Thiol caped gold NPs; Nanocomposite electrode;
Subjects: Chemistry
Polymer Science
Depositing User: Ms Neetu Chandra
Date Deposited: 15 Jun 2012 07:45
Last Modified: 15 Jun 2012 07:45

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