Das, Maumita and Sumana, Gajjala and R. , Nagarajan and Malhotra, B. D. (2010) Application of nanostructured ZnO films for electrochemical DNA biosensor. Thin Solid Films, 519 (3). pp. 1196-1201. ISSN 0040-6090

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Abstract

Nanostructured zinc oxide (nsZnO) films have been fabricated onto conducting indium–tin–oxide (ITO) coated glass plate, by cathodic electro-deposition to immobilize probe DNA specific to M. tuberculosis via physisorption based on strong electrostatic interactions between positively charged ZnO (isoelectric point = 9.5) and negatively charged DNA to detect its complementary target. Electrochemical studies reveal that the presence of nano-structured ZnO results in increased electro-active surface area for loading of DNA molecules. The DNA–nsZnO/ITO bioelectrode exhibits interesting characteristics such as detection range of 1 × 10-6 - 1 × 10-12 M, detection limit of 1 × 10-12 M (complementary target) and 1 × 10-13 M (genomic DNA), reusability of about 10 times, response time of 60s and stability of up to 4 months when kept at 4°C.

Item Type:	Article
Subjects:	Materials Science Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	16 Feb 2012 12:21
Last Modified:	27 Mar 2012 07:20
URI:	http://npl.csircentral.net/id/eprint/26

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