Kaushik, Ajeet and Solanki, Pratima R. and Kaneto, Keiichi and Kim, C. G. and Ahmad, Sharif and Malhotra, Bansi D. (2009) Nanostructured Iron Oxide Platform for Impedimetric Cholesterol Detection. Electroanalysis , 22 (10). 1045 -1055. ISSN 1521-4109

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A nanostructured iron oxide (NanoFe(3)O(4), particle size ca. 25 nm and roughness ca. 2:1 nm) film deposited onto a hydrolyzed indium-tin-oxide (ITO) coated glass plate has been used to immobilize cholesterol oxidase (ChOx) to fabricate an impedimetric cholesterol sensor. Electrochemical studies reveal that surface charged Fe(3)O(4) nanoparticles provide better conformation for ChOx loading resulting in enhanced electron transfer between ChOx and the electrode. Impedimetric response studies of the ChOx/NanoFe(3)O(4)/ITO bioelectrode exhibit improved linearity (2.5-400 mg/dL), low detection limit (0.25 mg/dL), fast response time (25 s), high sensitivity (86 Omega/mg dL(-1)/cm(-2)) and a low value of the Michaelis-Menten constant (K(m) 0.8 mg/dL) with a regression coefficient of 0.997.

Item Type: Article
Additional Information: Copyright for this article belongs to M/s Wiley-VCH Verlag Berlin.
Uncontrolled Keywords: Nanostrutured Fe3O4; Impedimetric biosensors; Cholesterol oxidase; Biosensors;
Subjects: Chemistry
Depositing User: Ms Neetu Chandra
Date Deposited: 13 Jun 2012 10:31
Last Modified: 13 Jun 2012 10:31
URI: http://npl.csircentral.net/id/eprint/254

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