Bandodkar, Amay Jairaj and Dhand, Chetna and Arya, Sunil K. and Pandey, M. K. and Malhotra, Bansi D. (2009) Nanostructured conducting polymer based reagentless capacitive immunosensor. Biomedical Microdevices , 12 (1). pp. 63-70. ISSN 1572-8781

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Abstract

Nanostructured polyaniline (PANI) film electrophoretically fabricated onto indium-tin-oxide (ITO) coated glass plate has been utilized for development of an immunosensor based on capacitance change of a parallel plate capacitor (PPC) by covalently immobilizing anti-human IgG (Anti-HIgG) using N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. These fabricated PANI/ITO and Anti-HIgG/PANI/ITO plates have been characterized using scanning electron microscopy, cyclic voltammetry, differential pulse voltammetry and Fourier transform infra-red studies. The capacitance measurements indicate that dielectric medium of this biologically modified PPC (Anti-HIgG/PANI/ITO) is sensitive to HIgG in 5− 5×105 ng mL−1 range and has lower detection limit of 1.87 ng mL−1. The observed results reveal that this Anti- HIgG modified PPC can be used as a robust, easy-to-use, reagentless, sensitive and selective immunosensor for estimation of human IgG.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Springer Verlag.
Uncontrolled Keywords:	Polyaniline; Electrophoretic deposition; Parallel plate capacitor; Immunosensor;
Subjects:	Biochemistry & Molecular Biology Electrochemistry Engineering
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	13 Jun 2012 10:19
Last Modified:	13 Jun 2012 10:19
URI:	http://npl.csircentral.net/id/eprint/253

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