Sharma, Aditya and Matharu, Zimple and Sumana, G. and Solanki, Pratima R. and Kim, C. G. and Malhotra, B. D. (2010) Antibody immobilized cysteamine functionalized-gold nanoparticles for aflatoxin detection. Thin Solid Films, 519 (3). pp. 1213-1218. ISSN 0040-6090
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Abstract
Aflatoxin B1 antibody (aAFB1) covalently attached to cysteamine functionalized-gold nanoparticles (C-AuNP) has been immobilized onto 4-mercaptobenzoic acid (MBA) based self assembled monolayer (SAM) on gold electrode (MBA/Au), for the fabrication of BSA/aAFB1-C-AuNP/MBA/Au immunoelectrode. This immunoelectrode has been characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and electrochemical characterization techniques. The electrochemical response studies reveal that the BSA/aAFB1-C-AuNP/MBA/Au immunoelectrode can be used to detect AFB1 in the range of 10–100 ng dL− 1 and has sensitivity as 0.45 μA ng− 1 dL, limit of detection as 17.90 ng dL− 1 and a response time of 60 s.
| Item Type: | Article |
|---|---|
| Subjects: | Materials Science Physics |
| Divisions: | UNSPECIFIED |
| Depositing User: | Ms Neetu Chandra |
| Date Deposited: | 16 Feb 2012 11:34 |
| Last Modified: | 27 Mar 2012 07:19 |
| URI: | http://npl.csircentral.net/id/eprint/25 |
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