Singh, S. and Solanki, P. R. and Pandey, M. K. and Malhotra, B. D. (2006) Cholesterol biosensor based on cholesterol esterase, cholesterol oxidase and peroxidase immobilized onto conducting polyaniline films. Sensors and Actuators B: Chemica, 115 (1). pp. 534-541. ISSN 0925-4005

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Abstract

Cholesterol oxidase, cholesterol esterase and peroxidase have been co-immobilized onto electrochemically prepared polyaniline films. These polyaniline–enzyme films characterized using spectroscopic techniques, have been used to fabricate a cholesterol biosensor. This polyaniline-based cholesterol biosensor has a response time of about 240 s, an apparent Km value as 75 mg dl−1 and can be used to estimate cholesterol concentration up to 500 mg dl−1. These polyaniline/cholesterol oxidase/cholesterol esterase films have a detection limit of 25 mg dl−1 with sensitivity of 0.042 μA mg dl−1. The enzyme films were found to be thermally stable up to 48 °C and have a shelf-life of about 6 weeks when stored at 4 °C. The values of the activation energy before and after the critical temperature were found to be 191 and 95.5 kJ mol−1, respectively.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier
Subjects:	Chemistry > Analytical Chemistry Electrochemistry Instruments/ Instrumentation
Divisions:	UNSPECIFIED
Depositing User:	Users 27 not found.
Date Deposited:	07 May 2018 08:50
Last Modified:	07 May 2018 08:50
URI:	http://npl.csircentral.net/id/eprint/2428

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