Ghrera, AdityaS harma and Pandey, Manoj Kumar and Malhotra, Bansi Dhar (2016) Quantum dot monolayer for surface plasmon resonance signal enhancement and DNA hybridization detection. Biosensors and Bioelectronics, 80. pp. 477-482. ISSN 0956-5663

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Abstract

We report results of studies relating to the fabrication of a surface plasmon resonance (SPR)-based nucleic acid sensor for quantification of DNA sequence specific to chronic myelogeneous leukemia (CML). The SPR disk surface has been modified with octadecanethiol self-assembled monolayer followed by deposition of the tri-n-octylphosphine oxide capped cadmium selenide quantum dots (QD) Langmuir monolayer. The deposition is performed via Langmuir-Blodgett (LB) technique. For the sensor chip preparation, covalent immobilization of the thiol-terminated DNA probe sequence (pDNA) using displacement reaction is accomplished. This integrated SPR chip has been used to detect target complementary DNA concentration by monitoring the change in coupling angle via hybridization. It is revealed that this biosensor exhibits high sensitivity (0.7859 m(0) pM(-1)) towards complementary DNA and can be used to detect it in the concentration range, 180 pM to 5 pM with detection limit as 4.21 pM. The results of kinetic studies yield the values of hybridization and dissociation rate constants as 9.6 x 10(4) M-1 s(-1) and 2.3 x 10(-2) s(-1), respectively, with the equilibrium constant for hybridization as 4.2 x 10(6) M-1.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Elsevier.
Uncontrolled Keywords:	Quantum dots Cadmium selenide Surface plasmonresonance Langmuir–Blodgett Nucleicacidhybridization Chronic myelogenousleukemia Biosensor
Subjects:	Biophysics Biotechnology & Applied Microbiology Chemistry > Analytical Chemistry Electrochemistry Nanoscience/ Nanotechnology
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	16 Apr 2018 08:39
Last Modified:	16 Apr 2018 08:39
URI:	http://npl.csircentral.net/id/eprint/2342

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