Ali , Md. Azahar and Solanki , Pratima R. and Patel, Manoj K. and Dhayani, Hemant and Agrawal, Ved Varun and John, Renu and Malhotra, Bansi D. (2013) A highly efficient microfluidic nano biochip based on nanostructured nickel oxide. Nanoscale, 5 (7). pp. 2883-2891. ISSN 2040-3364

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Abstract

We present results of the studies relating to fabrication of a microfluidic biosensor chip based on nickel oxide nanorods (NRs-NiO) that is capable of directly measuring the concentration of total cholesterol in human blood through electrochemical detection. Using this chip we demonstrate, with high reliability and in a time efficient manner, the detection of cholesterol present in buffer solutions at clinically relevant concentrations. The microfluidic channel has been fabricated onto a nickel oxide nanorod-based electrode co-immobilized with cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) that serves as the working electrode. Bare indium tin oxide served as the counter electrode. A Ag/AgCl wire introduced to the outlet of the microchannel acts as a reference electrode. The fabricated NiO nanorod-based electrode has been characterized using X-ray diffraction, Raman spectroscopy, HR-TEM, FT-IR, UV-visible spectroscopy and electrochemical techniques. The presented NRs-NiO based microfluidic sensor exhibits linearity in the range of 1.5–10.3 mM, a high sensitivity of 0.12 mA mM−1 cm−2 and a low value of 0.16 mM of the Michaelis–Menten constant (Km).

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/S Royal Society of Chemistry.
Subjects:	Chemistry Materials Science Applied Physics/Condensed Matter Nanoscience/ Nanotechnology Physics
Divisions:	UNSPECIFIED
Depositing User:	Ms Neetu Chandra
Date Deposited:	12 Mar 2018 10:51
Last Modified:	12 Mar 2018 10:51
URI:	http://npl.csircentral.net/id/eprint/2313

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