Gupta, Pramod K. and Gupta, A. and Dhakate, S. R. and Khan, Zishan H. and Solanki, Pratima R. (2016) Functionalized polyacrylonitrile-nanofiber based immunosensor for Vibrio cholerae detection. Journal of Applied Polymer Science, 133 (44). ISSN 0021-8995

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Abstract

Polyacrylonitrile nanofibers (PANnf's) were electrospun directly onto an indium tin oxide (ITO)-coated glass substrate. The PANnf/ITO electrode was partially hydrolyzed with an NaOH aqueous solution at ambient temperature to convert the nitrile groups of the PANnf's into carboxyl groups was confirmed by Fourier transform infrared spectroscopy. Furthermore, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxy succinimide chemistry was used to activate the ACOOH groups of PANnf's for the covalent co-immobilization of monoclonal antibodies against Vibrio cholerae and bovine serum albumin for V. cholerae toxin detection. Structural, functional, and electrochemical studies of the PANnf/ITO electrode and BSA/Ab/PANnf/ITO immunoelectrode were performed, and found a uniform distribution of nanofibers with diameter of 325+/-7.7% nm. The electrochemical response studies showed an improved sensing performance of the immunoelectrode with a detection of 6.25-500 ng/mL, a low limit of detection of 0.22 ng/mL, a sensitivity of 90 nA ng(-1) mL cm(-2), an association constant of 45.2 ng/mL, and a dissociation constant of 8 ng/mL.

Item Type:	Article
Additional Information:	Copyright for this article belongs to M/s Wiley.
Uncontrolled Keywords:	biomedical applications; electrospinning; nano fibers; Vibrio cholerae
Subjects:	Polymer Science
Divisions:	UNSPECIFIED
Depositing User:	Dr. Rajpal Walke
Date Deposited:	20 Nov 2017 11:19
Last Modified:	20 Nov 2017 11:19
URI:	http://npl.csircentral.net/id/eprint/2227

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